Background: Sepsis and surgical site infections (SSIs) are major causes of morbidity and mortality following traumatic injury. While prompt surgical intervention and antibiotics are critical, the role of protocolized, nurse-driven anti-infective care in the emergency setting remains underexplored. This study evaluated the effectiveness of a structured, multi-component anti-infective nursing bundle on clinical outcomes in emergency surgical trauma patients. Methods: A quasi-experimental, pre-post intervention study was conducted at a Level I trauma center. The control group consisted of 415 patients admitted between July and December 2022, receiving standard care. The intervention group consisted of 428 patients admitted between January and June 2023, managed with a novel "TIME" nursing bundle: Timely administration of antibiotics and cultures, Intensive metabolic control (glycemic & temperature), Meticulous wound and catheter care, and Early source control coordination. Primary outcomes were the incidence of sepsis within 72 hours and SSI within 30 days. Secondary outcomes included antibiotic days, length of ICU stay, and 30-day mortality. Data were analyzed using chi-square tests, t-tests, and multivariate logistic regression. Results: Implementation of the TIME bundle was associated with significant improvements. The sepsis rate decreased from 18.1% to 11.0% (p<0.01), and the SSI rate decreased from 12.3% to 7.0% (p<0.01). Patients in the intervention group had fewer mean antibiotic days (5.2 vs. 7.8, p<0.01), shorter ICU stays (4.5 vs. 6.1 days, p<0.05), and lower 30-day mortality (8.6% vs. 12.8%, p<0.05). After adjusting for injury severity, the TIME bundle was an independent protective factor against sepsis (aOR 0.52, 95% CI 0.34-0.79) and SSI (aOR 0.49, 95% CI 0.30-0.80). Conclusion: A structured, nurse-led anti-infective care bundle significantly reduced the incidence of early sepsis and surgical site infections in emergency trauma surgery patients, and was associated with improved antibiotic stewardship and clinical outcomes. These findings highlight the pivotal role of targeted nursing interventions in the multidisciplinary effort to prevent infections in trauma care.